Relationship of Human Sperm Acrosin and Proacrosin to Semen Parameters II. Correlations
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چکیده
منابع مشابه
Proacrosin-acrosin activity in capacitated and acrosome reacted sperm from cryopreserved bovine semen.
Acrosin activity is associated with normal fertility in human and bovine spermatozoa. The aim of the study was to determine the variation of the enzyme activity in the proacrosin-acrosin system in capacitated and acrosome reacted cryopreserved bovine sperm. Enzyme activity was assessed spectrophotometrically using N-alpha-benzoyl-DL-arginine p-nitroanilide (BAPNA) as specific substrate for acro...
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A proacrosin conversion inhibitor present in boar spermatozoa has been purified and initially characterized. Purification methods included sequential acid extractions of washed spermatozoa at pH 4.0, pH 3.5, and pH 2.5 followed by successive gel filtrations of the pH 2.5 sperm extract supernatant over Sephadex G-75 and G-50. The resulting 8.8-fold purified materials were judged to be homogeneou...
متن کاملRelationship between Semenogelins bound to human sperm and other semen parameters and pregnancy outcomes
BACKGROUND Semenogelins (SEMGs) are major components of human seminal vesicle secretions. Due to SEMG's sperm-motility inhibitor, a significant negative correlation between sperm motility and the proportion of SEMG-bound spermatozoa (SEMG+) was found in asthenozoospermic patients. SEMGs also show intrinsic inhibitory capability for sperm capacitation; however, studies on actual clinical specime...
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When proacrosin from mouse epididymal spermatozoa was activated a single form of acrosin was produced. The enzyme was isolated by gel filtration followed by affinity chromatography using Sepharose-4B linked to an acrosin inhibitor p-(p'-aminophenoxypropoxy)benzamidine. The molecular weight of partly purified acrosin was 53 000 by gel filtration, and of the pure enzyme 39 000 by SDS-polyacrylami...
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Background: Varicocele occurs in approximately 15% to 20% of the male population and it is the most common cause of poor semen parameters. It has been demonstrated that patients with varicocele have poor chromatin packing, DNA damage and nuclear anomalies than the healthy men. Therefore, the aim of this study was to evaluate sperm chromatin integrity and semen parameters in these patients after...
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ژورنال
عنوان ژورنال: Journal of Andrology
سال: 1987
ISSN: 0196-3635
DOI: 10.1002/j.1939-4640.1987.tb03320.x